Platform for protein crystallography and X-ray scattering

Platform for protein crystallography and X-ray scattering

About Facility

X-Ray scattering is the one of most powerful and the most frequently used method to obtain the proteins structure. The X-Ray Diffraction (XRD) on the protein crystals can give the information about protein structure with atomic resolution and the Small Angle X-Ray Scattering (SAXS) can give us the protein shape without crystallization, close to in-vivo state. The main problem of these methods is the low X-Ray contrast for biological objects that means you need an extremely powerful source of X-Rays. And we have it. Our X-Ray platform is based on Rigaku MicroMax-007HF microfocus rotating anode X-Ray generator with copper anode, X-Ray wavelength is 1.54Å.

Our expertise can be provided to support groups with no dedicated crystallographers at all stages from inception to final structure solution.

On the XRD part VariMax-HF confocal optical system installed and give us about 8×1010 particles/sec/mm2 on the 0.2-0.3mm diameter beam. Crystal holds on high-precise 4D goniometer with rotation axis about ω-axis and maximum possible resolution of 0.84Å and cooled with Oxford Cryosystems Cryostream with temperature up to 100K. Arcless goniometer provides housing for crystals in cryoloops and N2-cryostream maintains the cryoconditions. For detection of scattered photons Saturn 944HG cooled CCD detector is used, which have 2048x2048 pixels on 94x94mm imaging area. All of this give us the possibility to get the protein structure with resolution up to 1.5Å

For SAXS measurements we use the confocal Max-Flux SAXS optic with SMAXS-3000 pinhole system which form the X-Ray beam with 0.4mm diameter, about 10-4 rad angle divergence and flux about 4×108 particles/sec/mm2. Using of two sample chambers give us the q-range 0.005-1.2Å-1 which means possibility to detect objects about 5-1000Å size. Scattered photons are detected by extra-low noise gabriel-type gas detector with 1024×1024 pixels 200 mkm size. In addition sample environment have possibilities to do WAXS experiments, temperature control cell from -50 to 300℃ with +/- 0.1℃ precision, Grazing Incidence/Reflectivity module, Tensile stage and Shear cell.

Rigaku SAXS

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